Study on the Genetic Diversity of Natural Chestnut Populations in Shandong China by SSR Markers
The genetic diversity of 279 individuals from 10 populations in Shandong China was investigated using simple sequence repeat (SSR) markers. As a result, 79 bands were amplified by 12 informative and reliable primers, of which 71 were polymorphic loci. A relatively high level of genetic diversity was revealed: PPL=81.91, He=0.2698, Ho=0.3997 (at the species level); PPL=65.58, He=0.2004, Ho=0.3010 (at the population level). A higher level of genetic differentiation was detected among populations with Neis GST analysis and the analysis of molecular variance (AMOVA; GST=0.2441, FST=0.2206). Habitat fragmentation and gene flow may result in genetic differentiation. UPGMA cluster analysis indicated that the four populations from Linshu, Junan, Tancheng, and Feixian grouped together, whereas Laiyang populations clustered in an isolated clade. The results showed that a mixed mating system was possibly the main factor influencing the genetic structure of this species. These results, combined with other information about Castanea mollissima, may provide a valuable basis for proposing conservation strategies.
Chinese chestnut genetic diversity SSR Shandong province
Ai Cheng-xiang Li Guo-tian Zhang Li-si Liu Qing-zhong
Shandong Institute of Pomology Key Laboratory for Fruit Biotechnology Breeding of Shandong The Taian Key Field Observation Station for Temperate Fruit Resources of the Ministry of Agriculture Taian,Shandong,271000 China
国际会议
第四届国际板栗大会(Proceedings of Fourth International Chestnut Symposium)
北京
英文
257-266
2008-09-25（万方平台首次上网日期，不代表论文的发表时间）