Molecular Characterization of Hybrid Castanea Clones for Forest Reproductive Material Certification
TRAGSA applied RAPDs methodology to molecular characterize the Castanea hybrids clone collection that had been on propagation in its own nursery for years. Nowadays, basic materials of all forest Galician nurseries are being tested using this same method for its approval or refusement. Three different 10-mer primers (number 2 and 4 from kit A and number 11 from kit S, Operon Technologies) are necessary to properly identify the whole collection. The system is repetitive, and shows all the clones are different and present their own DNA profile. Therefore, this method is useful for the molecular characterization and certification of the new hybrid Castanea clones included in the Spanish Catalogue. At the same time, four SSRs markers have been tested with the same purpose, due to their high reproducibility and discrimination power.
RAPDs SSRs basic material ten-mer primers clonal identity
B.Cuenca L.González L.Oca(n)a M.Rey M.Lápez M.V.González
TRAGSA,Departamento de Mejora Agroforestal,Crta.Maceda-Valdrey,km.2,32700 Maceda,Ourense,Spain Departamento de Biologia Vegetal y Ciencia del Suelo,Universidad de Vigo,Facultad de Biologia,36301 Departamento de Fisiologia Vegetal,Universidad de Santiago de Compostela,Facultad de Biologia,15782
国际会议
第四届国际板栗大会(Proceedings of Fourth International Chestnut Symposium)
北京
英文
297-301
2008-09-25（万方平台首次上网日期，不代表论文的发表时间）