Determination of Mating Types of Cryphonectria parasitica Isolates Collected from Aydln Province/Turkey by Multiplez PCR
This study was conducted to determine the presence and distribution of the mating types, MAT-1 and MAT-2, and vc (vegetative compatibility) groups of Cryphonectria parasitica in the Aydm Province in Turkey. Total of 100 C parasitica isolates collected from the most affected nine chestnut growing villages of Nazilli, Sultanhisar, Kosk and Central Counties of the province were used in the study. Mating types of the isolates were determined by multiplex PCR assay with specific MAT-1 and MAT-2 primers. DNA fragments obtained from PCR were observed on electrophoresis and evaluated for their molecular lengths. The fragments which were 1.649 kb and 549 bp in length were identified as MAT-1 and MAT-2, respectively. Results indicated that 52％ of the isolates were MAT-1 and 48％ of MAT-2. Only two vc groups (EU-1 and EU-12) were detected.
hypovirulent vegetative compatibility vc group DNA MAT
S.A(c)ikg(o)z M.T.D(o)ken (O).Erincik Z.(O)zdemir N.F.De(g)irmenci
Faculty of Agriculture Department of Plant Pathology Adnan Menderes University Aydm Turkey Department of Plant Protection Office of Agricultural Affairs Manisa Turkey
国际会议
第四届国际板栗大会(Proceedings of Fourth International Chestnut Symposium)
北京
英文
367-372
2008-09-25（万方平台首次上网日期，不代表论文的发表时间）