Metabolic measurements in cell culture and tissue constructs
This paper concerns the study and use of biological cells in which there is a need for sensors and assemblies for the measurement of a diverse range of physical and chemical variables. In this field cell culture is used for basic research and for applications such as protein and drug synthesis, and in cell, tissue and organ engineering. Metabolic processes are fundamental to cell behaviour and must therefore be monitored reliably. Basic metabolic studies measure the transport of oxygen, glucose, carbon dioxide, lactic acid to, from, or within cells, whilst more advanced research requires examination of energy storage and utilisation. Assemblies are designed to incorporate bioreactor functions for cell culture together with appropriate sensing devices. Oxygen consumption by populations of cells is achieved in a flowthrough assembly that incorporates O2 micro-sensors based on either amperometry or fluorescence. Measurements in single cell are possible with intra-cellular fluorophores acting as biosensors together with optical stimulation and detection. Near infra-red spectroscopy (NIRS) is used for analysis within culture fluid, for example for estimation of glucose levels, as well as within cell populations, for example to study the respiratory enzymes.
Sensors bioreactors cell metabolism ozygen NIRS cellular engineering cell culture tissue regeneration
P Rolfe
Oxford BioHorizons Ltd, 25 Brewer St., Maidstone, Kent, ME14 1RU, England. Harbin Institute of Technology, 90 West Da-Zhi St., Harbin, China 150001. Advanced Research Institute for Science and Engineering, Waseda University, Tokyo, Japan
国际会议
第五届仪器科学与技术国际学术会议(ISIST 2008)Fifth International Symposium on Instrmentation Science and Technology
沈阳
英文
1-8
2008-09-15（万方平台首次上网日期，不代表论文的发表时间）