Digital pinhole filter used for detection of confocal microscopy images

摘要：

When conventional physical pinholes are used, the alignment of pinholes is always a problem. An iterative Wiener deconvolution filter is used to reject out-of-focus light, a sub-pixel centre location method is used to locate the centre of the pinhole, and the dimensions of the pinhole are optimized using the confocal microscopic theory. A filter with digital pinhole is thus developed and compared with filter with conventional physical pinhole through comparative tests. Experimental results indicate that the alignment of pinholes can be made easy, a filter with digital pinhole can be used to take place of a filter with conventional physical pinhole, and it can also reduce the pixel crosstalk resulting from multipoint illumination.

关键词： Virtual pinhole confocal out-of-focus light sub-pizel Wiener filter

作者: Xiangdong Huang Na Liu Junning Cui

作者单位: Harbin Institute of Technology Center of Ultra-precision Optoelectronic Instrument/No.2 YiKuangStree Harbin Institute of Technology Center of Ultra-precision Optoelectronic Instrument/No.2 YiKuang Stre

会议类型: 国际会议

会议名称: 第五届仪器科学与技术国际学术会议(ISIST 2008)Fifth International Symposium on Instrmentation Science and Technology

会议地点: 沈阳

会议语种:英文

页码: 1-5

在线出版日期: 2008-09-15（万方平台首次上网日期，不代表论文的发表时间）