Portable UV absorbance detection system for microchip electrophoresis
This paper presents a new structure of MCE chip on PMMA substrate prepared to improve the original chip structure with UV absorbance detection mode. The chip microchannels are fabricated by CNC milling and packaged with hot embossing process at the processing condition of 80, 5min, 0MPa with a manual powder pressing machine. All the micro-channels is 150μm * 150μm. Mobility μeo of PMMA MCE chip is 2 9.15e-004cm2/Vs in the solution of 10mmol/L Na2CrO4 and 0.5mmol/L CTAB. Adopting indirect UV photometric detection mode, the inorganic anion Clcapillary electrophoresis is carried out with an in-house UV detection system. The BGE buffer is 10 mmol/L Na2CrO4 and 0.5mmol/L CTAB, and the electrophoresis voltage is 616V. Test widow is 0.3*2 mm2. Test results indicate that both in-house UV detection system and MCE chip of PMMA substrate have the basic function and could be applied in the chemical analysis field.
micro-total-analytical-system (μ-TAS) microchip electrophoresis(MCE) PMMA substrate Ultraviolet absorbance spectroscopy (UV) detection photodiode computer numerical control (CNC) milling
Tian Li Wang Wei Liu Xiaowei Song Yu Lu Shaoyong
MEMS Center, Harbin Institute of Technology, Heilongjiang Province, China 150001
国际会议
第五届仪器科学与技术国际学术会议(ISIST 2008)Fifth International Symposium on Instrmentation Science and Technology
沈阳
英文
1-6
2008-09-15（万方平台首次上网日期，不代表论文的发表时间）