Sequence Analysis in Vicinity of Type 2 Diabetes Related SNP rs7903146
The SNP rs7903146 in human TCF7L2 gene has associated with type 2 diabetes (T2D) in multiple cohorts. TCF7L2 expression in human islets was increased in T2D, particularly in carriers of the TT genotype of SNP rs7903146. Over expression of TCF7L2 in human islets reduced glucosestimulated insulin secretion. The question of why the TCF7L2 genetic variants effect its selfs expression remained unanswered. We analyzed the sequence of about 20kb range in vicinity of rs7903146 SNP in combination with multiple bioinformatics programs in this study. We predicted 3 real pre-microRNAs on forward strand and 3 on reverse strand of 20kb vicinity sequence of rs7903146 respectively. Five potential promoters on forward strand and seven on reverse strand were also predicted. The TFSEARCH web server predicted the type 2 diabetes risk T allele of rs7903146 SNP has an E4BP4 binding site and two CDX1 binding sites which the common C allele has not. We hypothesized that E4BP4, CDX1 and some microRNAs in intron3 transcribed with host gene or independently in intron3 of TCF7L2 are involved in predisposing T2D byTCF7L2 gene variants.
Type 2 diabetes microRNA bioinformatics TCF7L2
JIAO Chuan-zhen TIAN Zhi-huan WEI Zhen-lin WANA Zai-zhao
Biology science department, DeZhou University DeZhou, P.R.CHINA, 253023
国际会议
上海
英文
50-53
2008-05-16（万方平台首次上网日期，不代表论文的发表时间）