Cloning and Sequence Analysis of a Disease Resistance Gene from wild Soybean seeds
The paper is to be in fully-justified italicized text, we have cloned some plant disease-resistant gene sequences in existence with some relatively Conserved domains. Conserved motifs in NBS domain have been used to isolate resistance gene analogs from soybean. NBS sequence and resistance gene sequence were used as probes to screen a wild soybean cDNA library, conserved motifs such as the nucleotide-binding site (NBS) were found in many characterized plant disease resistance genes. Resistance genes were used as probes to screen a wild soybean (Glycine soja.) cDNA library. A cDNA KR3-x (XLRR) was obtained and we used rapid amplification of cDNA ends (RACE) method from cDNA library and carried out bioinformatics analysis.
wild soybean disease resistance gene bioinformatic
SHI Ming-Wang WANG Qing-lian WANG Biao-zhong
Henan Institute of Science and Technology, Xinxiang city, 453003, Peoples Republic of China School of the Environment,Nanjing University Jiangsu,210003, NanJing city, China
国际会议
上海
英文
133-135
2008-05-16（万方平台首次上网日期，不代表论文的发表时间）