Effects of osthole on protein ezpression of CYP7A1, DGAT2 and PPARα/γ in fat milk-induced fatty liver rats
Aim: To determine the effect of osthole on protein expression of PPARα/γ and related target molecules such as CYP7A and DGAT in the liver of hyperlipidemic fatty liver (HFL) rats, and to investigate the possible mechanism of treating HFL. Methods: HFL rat model was established by feeding the Sprague-Dawley rats with fat milk for 6 weeks. The experimental rats were then treated with 5-20 mg/kg osthole for 6 weeks. The proteins expression of PPARα/γ, CYP7A1, and DGAT2 in rat liver was measured by western blot analyses. Results: After administration of osthole, expression of PPARα and PPARγ proteins in HFL rat liver was increased by 49％～133％ and 19％～113％ in the osthole groups as compared with model group, respectively (P<0.05 or P<0.01). The related target proteins were increased by 85％～147％ for CYP7A1 and decreased by 35％～62％ for DGAT2 by the osthole-treatment, respectively (P<0.01). Conclusions: Osthole possessed therapeutic effects on fat milk-induced fatty liver in rats, and the mechanism might be attributed to regulating the protein expression of CYP7A1 and DGAT2 via increasing PPARα/γ.
Osthole Hyperlipidemic fatty liver PPARα/γ DGAT2 CYP7A1
ZHANG Yan XIE Mei-lin XUE Jie GU Zhen-lun
Department of Pharmacology, Medical School of Soochow University, Suzhou 215123, China Wuxi Hospital Department of Pharmacology, Medical School of Soochow University, Suzhou 215123, China
国际会议
上海
英文
237-244
2008-05-16（万方平台首次上网日期，不代表论文的发表时间）