ISOLATION, PURIFICATION AND SOME PROPERTIES OF ALKALINE PHOSPHATASE FROM Hypophthalmichthys molitriz Cuv. et Val

摘要：

Abstracted by Tris-HCl buffer,pH8.5, treated by n-butanol, and fractioned by (NH4)2SO4 60％, the alkaline phosphatase(AKP) from guts of Hypophthalmichthys molitrix Cuv.et Val was seperated through DEAE-Sepharose and Sephacryl S-200.The purification folds was 150.08,while the specific activity was 4031.15 U/mg.The properties reasearch suggested that the optimal pH and temperature were pH10.2 and 40 respectively, and the AKP was unstable when the pH was out of range of pH7 to pH12, or the temperature was higher than 50.The data showed the Km value of the AKP was 4.73mmol/L.Also,some ions had effect on the activity of AKP,such as Mg2+which actvited the AKP and Zn2+ which inhibited the AKP.

关键词： Alkaline phosphatase Isolation Purification Properties Hypophthalmichthys molitriz Cuv.et Val

作者: TANG Yun-Ming Xu Min Ma Yao Du Hong-xuan Cheng Rong-chan Yang Qin

作者单位: School of Life Science, Southwest China University, Chongqing, china 400715

会议类型: 国际会议

会议名称: The 2nd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2008)(第二届生物信息与生物医学工程国际会议)

会议地点: 上海

会议语种:英文

页码: 282-285

在线出版日期: 2008-05-16（万方平台首次上网日期，不代表论文的发表时间）