Recognition and analysis of a Host-Targeting Motif in the genome of Fungus Magnaporthe grisea
(0) A highly conservative host-targeting motif composed of 4 amino acid residues, namely, RxLx 小 was reported to be present only in virulence proteins of malarial parasites (Plasmodium species) and the oomycete avirulence(Avr) protein of Phytophthora. In the present study, we analyzed the secretory proteins of the rice blast fungus Magnaporthe grisea that were predicted by bioinformatics and comparative genomic tools to determine whether the RxLx motif is present in the secretory proteins. In 297 of 1270 secretory proteins, the RxLx motif was detected within a region of 100 amino acids downstream the N-terminal signal peptide cleavage sites. A comparison with the PEDANT database revealed that RxLx motif-containing proteins included enzymes for the degradation of plant cell wall components such as cellulose, endoxylanase, endoglucanase and cutinase hydrolase for degradation of plant cell membrane and cellular contents, this suggests that the RxLx motif-containing proteins probably functioned as virulence proteins and were primarily involved in interaction between the rice blast fungus and host cells. So, our results support the idea that the host -targeting motif (HTM) is a conserved transport machinery, which imply that divergent eukaryotic pathogens might share common mechanisms of pathogenicity. This research will provide useful materials to elucidate whether similar or distinct pathogenic secretion signlals in different eukaryotic pathogens andy can helpful to validate predicted function of the HTM in vivo.
Magnaporthe grisea secretory proteins host-targeting motif pathogenicity
Ma Li Su Yuan Yang Jing Liu Lin Zhang Yue Kong Chui-si Yu Qing-liang Zhu You-yong Li Cheng-yun
The Ministry of Education Key Laboratory for Agricultural Biodiversity for Pest Management Yunnan Agricultural University,Kunming, China
国际会议
上海
英文
286-292
2008-05-16（万方平台首次上网日期，不代表论文的发表时间）