Agarose Gel Electrophoresis and Fluorometric Assays for the Determination of DNA Cross-linking Induced by Semustine
Semustine (Me-CCNU, 1-(2-chloroethyl)-3-(4-transmethylcyclohexyl)-1-nitrosourea) is significant anticancer agents in the clinical treatment of human malignancies. Several lines of evidences indicate that its cytotoxic activity is related to the interstrand cross-linking of DNA. In this study, the agarose gel electrophoresis and fluorometric assay were carried out to detect the NDA interstrand cross-linking caused by semustine. It was found that the cross-links of DNA induced by drugs increased progressively with the reaction time, tending towards a maximum stable value at about 8h. Simultaneously, a buffering stage in the beginning of DNA cross-linking was apparent. The results suggest that the formation of DNA interstrand crosslinking may undergo an induction period. It is proposed that the generation of DNA interstrand cross-linking go through two steps and is affected by the concentration of drugs.
Alkylating agents DNA Cross-linking Semustine Induction period
Wei Tang Li-Jiao Zhao Ru-Gang Zhong
College of Life Science and Bioengineering Beijing University of Technology Beijing 100022, China
国际会议
上海
英文
322-325
2008-05-16（万方平台首次上网日期，不代表论文的发表时间）