Repression of Nrf1-mediated Transactivation by MCRS2
Nrf1 p45 nuclear factor-erythroid 2 (p45 NF-E2)-related factor 1, a member of the CNC-bZIP (CNC basic region leucine zipper) family, is known to be a transcriptional activator via dimerization with distinct partners, such as Maf, FosB, c-Jun, JunD, etc. To find other proteins interacting with the CNC bZIP domain, the CNC-bZIP region of Nrf1 was used as a bait in a yeast two-hybrid screening. MCRS2, a splicing variant of p78/MCRS1, was isolated as the Nrf1-interacting partner. The interaction between Nrf1 and MCRS2 was confirmed in vitro by GST pull-down assays and in vivo by co-immunoprecipitation. The Nrf1-MCRS2 interaction domains were mapped to be the residues 354-447 of Nrf1 and the residues 314-475 of MCRS2 respectively by yeast twohybrid and GST pull-down assays. To further confirm the biological relevance, a reporter driven by CNC-bZIP protein binding sites was also shown to be repressed by MCRS2 in transient transfection assay. And a reporter gene activated by LexA-Nrf1 was specifically repressed by MCRS2. Taken together, these results pointed to a new Nrf1-interacting protein MCRS2 that can mediate repression effect on Nrf1-mediated transcriptional activation. Until now, this is the first repressor protein on Nrf1 transactivation activity and there is no other repressor (s) reported before.
Nrf1 MSP58 MCRS1 MCRS2 CNC-bZIP family
Jia-Long Wu Yu-Jen Lin Shan-Fu Wu Ying-Ting Lin Young-Sun Lin Chien-Fu Huang
IBMS Academia Sinica Taipei 11529, Taiwan Department of Fashion Design & Management Tainan University of Technology, Tainan, Taiwan Department of Biotechnology, College of Life Sciences line 2: Kaohsiung Medical University Kaohsiung Deputy Vice President Development Center for Biotechnology Taipei 11529, Taiwan Department of Biological Science and Technology I-Shou University Kaohsiung, Taiwan
国际会议
上海
英文
391-394
2008-05-16（万方平台首次上网日期，不代表论文的发表时间）