Relationship between Caspase-3 Ezpression and Apoptosis in Lung Adenocarcinoma Cell A-549 with Adenovirus-mediated FasL Gene
Investigate the relationship between caspase-3 expression and apoptosis in lung adenocarcinoma cell line A-549 who was transfected with Adenovirus-mediated FasL gene. Human lung adenocarcinoma cell line A-549 was transfected with Adenovirus-mediated FasL gene. The expression of caspase-3 apoptosis morphological changes were measured using flow cytometry, RT-PCR assays, the activity of caspase-3 and clevage of poly ADP-ribose polymerase(PARP) were determined with Western blot analysis. The expression of caspase-3 was low in lung adenocarcinoma cell line A-549, but transfection of lung adenocarcinoma cells with Ad-FasL enhanced cell expression of caspase-3 gene and induced susceptibility to Fas/FasL mediated apoptosis(44.08％ vs 18.15％, p<0.01). Apoptosis had positive relationship with the activity of caspase-3 (γ=0.647, p<0.01). Down-regulation of caspase-3 expression and resistance to Fas/FasL-mediated apoptosis in lung adenocarcinoma cells were found to be related to the development and progression of lung adenocarcinoma cells. FasL gene transfer may increase caspase-3 in lung adenocarcinoma cells and enhance their apoptosis.
membrane glycoproteins caspases Lung neoplasms/pathology oncogenes
Shi-ying ZHENG Hong LI Dong JIANG Jin-feng GE
Department of Thoracocardiac Surgery, The First Affiliated Hospital of Suzhou University,Suzhou, Jia Department of Geriatrics, The First Affiliated Hospital of Suzhou University,Suzhou, Jiangsu, 215006
国际会议
上海
英文
423-428
2008-05-16（万方平台首次上网日期，不代表论文的发表时间）