Cloning of Trehalose-6-Phosphate Phosphatase and Transformation to Tobacco
The trehalose-6-phosphate phosphatase (TPP) is one of enzymes related to trehalose biosynthesis. But the researches of TPP were mainly focused in microorganism; few in the plant were reported. In this study, the TPP gene was cloned from Arabidopsis seedling by using RT-PCR (reverse transcription PCR) method. Then it was constructed to pET30a (+) vector and overexpressed in E.coli. Subsequently, the TPP was ligated into the plant expressional vector pBI121 and transformed to tobacco. Three transgenic individuals have obtained and one of them has transcribed successfully through Real-time QPCR detection. The trehalose content has less visible increase in P3 individual, but there was no obvious difference between the transgenic plant and control under salt stress.
trehalose trehalose-6-phosphate phosphatase realtime QPCR HPLC tobacco
Guo Bei Jiang Xiang-ning
dept. of biotechnology Beijing university of agriculture Beijing, China college of life sciences and biotechnology Beijing forestry university Beijing, China
国际会议
上海
英文
441-444
2008-05-16（万方平台首次上网日期，不代表论文的发表时间）