2D DIGE Data Analysis for Differential Protein Spot Identification
Differential in-gel electrophoresis (DIGE) technique has been used for differential protein analysis to improve the reproducibility of comparative 2D gel experiments. Because the sample size in 2D DIGE experiments is usually very small, the traditional statistical methods such as student t-test could not provide an accurate and reliable detection/identification of differentially expressed protein spots. This paper proposed a ranking based approach for DE protein spot identification, in which the information of comparative ranks of the protein spot volume ratio rather than their distribution were used in selection. Two methods were designed to integrate the ranking information from a set of pair-wise comparisons. The evaluation results demonstrated that the proposed methods are effective in DE protein spot identification when sample size is small.
protein DIGE detection identification Ranking
Lihua Li Lei Zhu Bin Han Li Chen Rebecca Sutphen
Institute for Biomedical Engineering & Instrumentation, Hangzhou Dianzi University Hangzhou, China Department of Electrical and Computer Engineering, Virginia Polytechnic Institute and State Universi H. Lee Moffitt Cancer Center & Research Institute University of South Florida, USA
国际会议
上海
英文
620-623
2008-05-16（万方平台首次上网日期，不代表论文的发表时间）