Using fluorescent-based AFLP to analyze genetic diversity of yellow-seeded Brassica napus L.
A method for multiplex AFLP-PCR was employed to access the genetic diversity of yellow-seeded Brassica napus accessions originated from China, Czech Republic, and Poland. The AFLP-PCRs using three blended and fluorescent-labeled EcoRI+3 primers, AAC, AAG, and ACA, combing each time with one MseI+3 primers CTC, CAC, CAG, CTG, CTT, and CTA, totally generated 723 peaks, of which 632 had high intension. 242 distinctly polymorphic markers among them were scored, with an average of 13.4 markers per primer pair. Based on either Dice, Jaccard, or Hamman similarity coefficients, the 31 accessions tested could be separated into Chinese, and non-Chinese group by UPGMA clustering, which corresponding well to the origins and pedigrees. Distinct geographical divergence was found among Chinese and non-Chinese yellow-seeded accessions. This AFLPs protocol were shown to be a reliable and quickly method for analyzing the genetic diversity than numeric taxonomy methods.
AFLP multiplez PCR genetic diversity Brassica napus yellow-seeded
Chengyu Yu Leona Leisova Vratislav Kucera Miroslava Vyvadilova Jaroslava Ovesna Shengwu Hu Ladislav Dotlacil
College of Agronomy, Northwest A&F University, Yangling, 712100, China Crop Research Institute, 16106, Prague 6, Czech Republic
国际会议
上海
英文
714-717
2008-05-16（万方平台首次上网日期，不代表论文的发表时间）