Microbial Diversity Research of Produced Water from Daqing Oil Reservoir after Polymer Flooding
DNA was extracted from four oil well samples in Daqing oil reservoir after polymer flooding. A 16S rDNA clone library was constructed by PCR amplification applying universal primers, cloned in E.coli and subsequently characterized by amplified rDNA restriction analysis (ARDRA). 298 clones were obtained and classified into 22 different operational taxonomic units (OTUs). Four OTUs occupied 78.18 percent of the 16S rDNA clone library. Nine OTUs have only one clone. At the same time, the microbial diversity of the 16S rDNA clone library was evaluated by Coverage value (C), Shannon-Weiner index (H) and Evenness index (E). Phylogenetic analysis showed that the dominant members of the library were affiliated with the Alphaproteobacteria (20.47％), the gammaproteobacteria (23.81％), the Deltaproteobacteria (32.21％) and the uncultured bacteria (21.16％). Pseudomonas and Acinetobacter which have the connection with biodegradation of hydrocarbon and production of surfactant occupied 17.79％ and 6.02％ in the clone library, respectively.
amplified ribosomal DNA restriction analysis (ARDRA) oil reservoir after polymer flooding microbial diversity 16S rDNA clone library
She Yuehui Zhang Xuemei Zhang Fan Wang Zhengliang Shu Fuchang Zhou Lingge Xiang Tingsheng Yu Longjiang
College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, College of Chemical Engineering, China University of Petroleum, Beijing 102249, China College of Chemical and Environmental Engineering, Yangtze University, Jingzhou 434023, China College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074,
国际会议
上海
英文
771-775
2008-05-16（万方平台首次上网日期，不代表论文的发表时间）