Biological Evaluation of Porous Chitosan/collagen Scaffolds for Periodontal Tissue Engineering
Porous chitosan/collagen scaffolds were investigated via biological evaluation to learn whether it could be used for periodontal tissue engineering. Periodontal ligament cells (PDLCs) were co-cultultured with chitosan/collagen scaffold and cytocompatibility were evaluated by MTT assay, as well as SEM and CLSM observation. PDLCs mRNA expression of ECM was carried out by RT-PCR tests. For tissue compatibility evaluation, scaffolds were implanted subcutaneously under the skin of rabbits ears and HE staining was used. The delivery ability was also taken by release test of BSA and plasmid DNA from scaffold. The results revealed that PDLCs could adhere and grow well on scaffold. Compared to plate culture, PDLCs showed similar mRNA express level of ECM and higher cell capacity. The scaffold showed good tissue compatibility and degrade completely in vivo after 6 weeks of implantation. Such scaffold also could behave as control delivery system of protein and DNA. So chitosan/collagen was a kind of promising scaffold material for periodontal tissue engineering.
chitosan/collagen scaffold PDLCs tissue engineering cytocompatibility tissue compatibility
Lin Peng Ren-Xi Zhuo
College of Chemistry and Molecular Sciences, Wuhan University Key Laboratory of Biomedical Polymers, Ministry of Education, Wuhan University. Wuhan, 430072 P.R.China
国际会议
上海
英文
897-900
2008-05-16（万方平台首次上网日期，不代表论文的发表时间）