Somatic embryogenesis from hairy roots transformed by Agrobacterium rhizogenes in Panaz ginseng

摘要：

An efficient in vitro protocol for somatic embryogenesis of hairy roots of Panxa ginseng has been established. Hairy roots of Panax ginseng were obtained after root disks were infected with Agrobacterium rhizogenes A4. Callus was induced on MS (Murashige and Skoog 1962) medium containing 2,4-D (2,4-Dichlorophenoxyacetic acid), BA (6-Benzyl-aminopurine), Kin (Kinetin). The highest frequency of callus formation was 99.3％ on MS medium supplied with BA and Kin and those callus have fewer secondary roots. Embryogenic callus was efficiently achieved by lowering the concentration of plant growth regulators. The frequency of somatic embryogenesis from hairy roots on MS medium with 0.5 mg 1-1 2,4-D and 0.1 mg 1-1 Kin was up to 42.7％. Embryogenic callus can further develop to germinate on MS basal medium supplemented with different concentration GA3 and the highest germination was obtained on medium with 5 mg 1-1 GA3 (Gibberellic acid).

关键词： Panaz ginseng Hairy root Somatic embryogenesis

作者: Wang Jianhua Zhao Shoujing Xue Jian Xu Lixin Hou Chunxi Liang Yanlong

作者单位: School of Biological and Agricultural Engineering, Jilin University, Changchun, China

会议类型: 国际会议

会议名称: The 2nd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2008)(第二届生物信息与生物医学工程国际会议)

会议地点: 上海

会议语种:英文

页码: 911-914

在线出版日期: 2008-05-16（万方平台首次上网日期，不代表论文的发表时间）