NMDA-receptor-initiated modulatory effect on GABA transporter current: ezperiment and modelling
Whole cell recording was applied to investigated the modulatory effect of N-methyl-D-aspartate (NMDA) on γ-aminobutyric acid (GABA) transporter current on enzymatically isolated horizontal cells of carp retina, and a model containing endoplasmic reticulum (ER) membrane process and plasma membrane process was constructed for quantitative analyses of the modulatory effect of NMDA and changes of intracellular Ca2+. The GABA transporter current elicited by GABA (1 mM) was decreased by pre-application of NMDA (0.1 mM). The suppressive effect could be attenuated when the intracellular Ca2+ store was depleted after the cell had been pre-incubated with 10 μM thapsigargin, a selective blocker of ER Ca2+-ATPase. The model analyses suggest that application of NMDA caused a transient release of Ca2+ from Ca2+ store, which was important for the NMDA modulation of GABA transporter.
NMDA receptor calcium store GABA transporter horizontal cell
Xiao-Dong Jiang Xu-Long Wang Yan Sun Hai-Qing Gong Pei-Ji Liang
Department of Biomedical Engineering Shanghai Jiao Tong University Shanghai 200240, China
国际会议
上海
英文
975-978
2008-05-16（万方平台首次上网日期，不代表论文的发表时间）