Determination of Xylidine Ponceau 2R in Red Chilli Pepper by HPLC With Solid Phase

摘要：

A quantitive assay for the determination of Xylidine Ponceau 2R was established by high performance liquid chromatography (HPLC) with solid phase extraction. Xylidine Ponceau 2R was extracted by alcohol-ammonia, solid phase extraction was utilized to cleanup. The separation was performed in a XDB-C18 column, acetonitrile, 5mmol/L ammonium acetate and 0.06％ triethylamine as mobile phase. A photo-diode array detector was used to detect the compositions at 504 nm. The linear range of the method was 0.1~15μg/mL, the limit of detection was 0.02mg/L, the relative standard deviation was 0.33-2.86％ and the average recoveries were 90.66～93.89％. The method was accuracy, sensitivity and feasibility to analyze the residues of Xylidine Ponceau 2R in foodstuffs.

关键词： Xylidine Ponceau 2R HPLC solid phase eztraction

作者: Zhilei_Zhao Xiaoting_Li Guangchen_Wu

作者单位: College of Quality & Technical Supervision Hebei University, Baoding,China

会议类型: 国际会议

会议名称: The 2nd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2008)(第二届生物信息与生物医学工程国际会议)

会议地点: 上海

会议语种:英文

页码: 1087-1089

在线出版日期: 2008-05-16（万方平台首次上网日期，不代表论文的发表时间）