Feed Strategies for improving ezpression of nonmitogenic form of human acidic fibroblast growth factor in Escherichia coli
Non-mitogenic form of Human acidic fibroblast growth factor (nhaFGF) retained the cardio-and neuroprotective characters of the wild-type aFGF while decreased the potential side effects like mitogenesis activity. In this paper, the effect of acetate concentration on the growth and expression of recombinant nhaFGF system Escherichia coli BL21 (DE3) /pET3C-nhaFGF was investigated. Four fedbatch modes: fed-batch, batch-static DO balance, static DO balance-glucose starvation, and pH-static state were investigated. The accumulation of acetate during the fermentation course was effectively inhibited. The OD600nm value was about 22, after purification, the soluble rhaFGF yielded 450mg/L. During the fermentation, no special ways such as pure oxygen, pressure were adopted, thus the established process would be easily scaled up for industry purpose.
Acidic fibroblast growth factor Escherichia coli fed-batch static DO balance glucose starvation pH static state
Hui YUAN Xiao Kun LI
Zhejiang University of Science and Technology Key Laboratory of Agricultural Products Chemical and B Wenzhou Medical College Bio-natural Pharmaceutiacl School Wenzhou, P.R.China,325035
国际会议
上海
英文
1098-1102
2008-05-16（万方平台首次上网日期，不代表论文的发表时间）