Fingerprinting technique of Chinese Traditional Medicine and Its Applications
The optimal conditions for extraction, isolation and determination of chemical components of Gastrodia tuber with HPLC (High-performance liquid chromatography) were established. Using these conditions the chemical fingerprints and Gastrodin content of Gastrodia tuber samples from 6 different provinces or regions were determined. The results show that the chemical fingerprints of all tested Gastrodia tubers have five common peaks with very approximate retention time. The chemical fingerprints of Gastrodia tuber from the same province are quite similar, but those of Gastrodia tubers from the different provinces are not. Several better Gastrodia tuber populations were found with higher gastrodin content. The findings can be used to identify the quality of Gastrodia tuber, authenticate genuine Gastrodia tuber, determine the Gastrodine content and discover the better Gastrodia tuber populations. The same techniques may be applied to similar research of other medicinal plants.
Gastrodia tuber HPLC Chemical fingerprints Gastrodin content Quality identification parameter optimization
Jun Tao Yan Wang Ping Chen Zhicheng Wang Li Wen Xuewei Jiang Xiao Hu
School of biology and food engineering, Changsha university of science & technology, Changsha, P.R. China
国际会议
上海
英文
1108-1111
2008-05-16（万方平台首次上网日期，不代表论文的发表时间）