The effect of solanine on the membrane potential of mitochondria in HepG2 cells and Ca2+i in the cells
To observe the effect of solanine on the membrane potential of mitochondria in HepG2 cells and Ca2+i in the cells, and to uncover the mechanism by which solanine induces apoptosis.HepG2 cells are double stained with TMRE and Fluo-3/AM, and both the change in membrane potential of mitochondria and that of Ca2+i in the cells are observed using LCSM.The results of double staining with TMRE and Fluo-3/AM show that solanine can lower membrane potential and increase the concentration of Ca2+ in the cells Solanine opens up the PT channels in the membrane by lowering the membrane potential, leading to Ca2+ being transported down its concentration gradient, which in turn leads to the rise of the concentration of Ca2+ in the cell, turning on the mechanism for apoptosis.
Solanine Hepatocarcinomatic cell Ca2+ in the cell Membrane potential Laser confocal scanning microscopy (LCSM)
JI Yu-Bin GAO Shi-yong JI Chen-Feng ZOU Xiang YU Lei
Institute of Meteria Medica,Center of Research and Development on Life Sciences and Environment Sciences Harbin University of Commerce Harbin, China
国际会议
上海
英文
1153-1156
2008-05-16（万方平台首次上网日期，不代表论文的发表时间）