Pyrolysis- GC/MS Analysis of Biomedical Constituents in the Acetone Eztractive of Osmanthus fragrans Leaf by Ultrasonic Eztraction
As the noble gardening tree growing specifically in China, Osmanthus fragrans is widely used to produce high-grade essence, special drugs and aromatic tea form its flowers. However, O. fragrans leaves are still underutilized and hence wasted. Therefore, in order to better utilize O. fragrans leaves and hence to expand its utility values and industrial scopes, we attempted to analyze the bioactive components, especially biomedical constituents from O. fragrans leaf by the way of Py-GC/MS (Pyrolysis-gas chromatograph/ mass spectrometer). After acetone extraction on O. fragrans fresh leaf by ultrasonic extraction for 5 h at 90℃, and with the removal of solvent, the extractive was pyrolyzed in 590℃ He atmosphere, then the pyrolysis product was analyzed by online linked GC/MS. Relative content of each component was determined by area normalization, and 64 compounds representing 97.85％ of the acetone ultrasonic extractive were identified from 65 peaks (Figure 1). The main constituents were as: friedelan-3-one (38.87％), lup-20(29)-en-3-one (12.46％), Stigmasterol,22,23-dihydro-(6.23％), Urs-12-ene (5.45％), benzoic acid,4-hydroxy-3-methoxy-(2.08％), stigmasterol (1.96％), cyclotetracosane (1.92％), acetone (1.59％), beta-amyrin (1.57％), benzoic acid,4-hydroxy-(1.32％), 1,6-anhydro-.beta.-D-glucopyranose (1.32％), toluene,3-(2-cyano-2-phenylethenyl-) (1.22％), taraxasterol (1.20％), 1,2-propanediamine (1.07％), hexadecanoic acid (1.00％), ergosta-4,6,22-trien-3.alpha.-ol (1.00％), 1,2-benzenediol (0.91％), vitamin E (0.90％), campesterol (0.89％), etc. The results of function analyses suggested that the pyrolysis product from the acetone ultrasonic extractive of O. fragrans leaf is abundant in materials of noble natural medicines, and also contains constituents which can be developed into top valueadded materials of cosmetics, biofuel and industrial. This is the first report here that showed underutilized leaf of O. fragrans contains abundant biomedical constituents and can be developed into top value-added products.
Osmanthus fragrans leaf biomedical constituents Pyrolysis- GC/MS (Pyrolysis- gas chromatograph/mass spectrometer) top value added ultrasonic eztraction
Qi-Mei LIU Yin-She LUO Hong WANG Wan-Xi PENG Sheng-Ming CHEN Min YU Dang-Quan ZHANG Qing-Zhi MA
Institute of Rheological Mechanics and Material Engineering, Central South University of Forestry an Non-wood Forest Products of State Forestry Administration, Central South University of Forestry and
国际会议
上海
英文
1217-1220
2008-05-16（万方平台首次上网日期，不代表论文的发表时间）