Preparation of Nano-sized Collagen Ⅰ and Collagen Ⅱ Particles
This pilot study describes a new method to prepare nano-sized collagen type Ⅰ and collagen type Ⅱ particles by using a high-voltage electrostatic field system. Observations from transmission electron microscopy showed that, the nano-sized collagen II particles with a range of 150 nm in diameter could be produced with a good spherical shape under the experimental setting of 2.7～4.5 kVcm-1, under 23℃ and a moderate concentration of collagen Ⅱ solution at 0.2 mg/ml. When the treatment temperature increased to 30℃ or higher, collagen Ⅱ began to lose the tendency to individually separated spherical shaped nano-sized particles, instead, the fibrillogenesis of collagen Ⅱ occurred by the spontaneous reconstitution of collagen Ⅱ and then formed collagen Ⅱ fibrils. More strict experimental settings were necessary for preparing nano-sized collagen I particles, that are, lower temperature setting (under 7℃ environment), lower collagen concentration (0.1 mg/ml) and lower applied voltage (2.5 kVcm-1). Moreover, these prepared nano-sized collagen I particles exhibited smaller size in diameter (100 nm) but poorer sphericity than collagen Ⅱ particles. They often aggregated together and exhibited an irregular outer shape.
Shyh Ming Kuo Shwu Jen Chang Ioannis Manousakas Li Chun Lin Ting Wei Liu
Department of Biomedical Engineering, I-SHOU University, Taiwan Meiho Institue of Technology, Taiwan
国际会议
上海
英文
1417-1419
2008-05-16（万方平台首次上网日期，不代表论文的发表时间）