Alginate-templated microcapsules carrying heparin via layer-by-layer self-assembly
Electrostatic layer-by-layer self-assembly is a simple and effective approach to carry macromolecules into microscale carriers. Calcium-cross-linked alginate hydrogel microspheres were fabricated by an emulsification technique and then used as negatively charged templates for polyelectrolyte multilayer coatings to entrap heparin. The polycation was poly (allylamine hydrochloride) (PAH), while the polyanion was heparin, an anticoagulant to prevent the clotting of blood. The microcapsule was formed via coating PAH/heparin3 on Ca2+-cross-linked alginate hydrogel. The retention of alginate in PAH/heparin3 microcapsule was confirmed by fluorescence inverted microscop and confocal laser scanning microscopy. The assembly of polyelectrolyte layers on alginate microspheres was monitored by Zeta Potential Analyzer. The concentration of heparin was detected by HPLC with IR-detector and organic acid column. It was found that heparin could be entrapped in the microcapsules in high efficiency, providing a useful delivery system which would be found potential application in antagonizing thrombosis.
Alginate Heparin Layer-by-Layer self-assembly Microcapsules
Juan Li Xinrong Liu Baoqing Zhu Yingya Shao Dongxu Zhao Xinlin Yang
School of Life Science and Technology, Beijing Institute of Technology, Beijing, China, 100081
国际会议
上海
英文
1420-1423
2008-05-16（万方平台首次上网日期，不代表论文的发表时间）