Thermal Gradient Instrument for Gene Amplification
This paper presents an integrated thermal gradient device for gene amplification. This instrument consists of a 16-bit MCU chip with 256k flash-ROM, six thermoelectric heat pumps placed on top of a micro-machined silicon chip integrated with three temperature sensors, three power amplifiers, three integrated 12-bit analog-to-digitals, three integrated 12-bit digital -to-analogs and a lid-heater. It is a new gene amplification technique that is characterized by periodic temperature ramping. There are three channels that are determined by temperature control system individually in the instrument. The advantages of this system are the high stability of the temperature, rapid thermo-cycling and the easy of establishment of the adequate conditions for the nested polymerase chain reaction.
PCR gene amplification temperature control
Jinhe Wang
School of Computer Engineering Qingdao Technological University Qingdao, Shandong, China
国际会议
上海
英文
1472-1475
2008-05-16（万方平台首次上网日期，不代表论文的发表时间）