Biosensing with Localized Surface Plasmon Resonance Based on Cap-shaped Gold Nano-particles
In this paper, the optical biosensors based on capshaped gold nano-particles have been constructed by evaporating gold on polystyrene and silica microspheres to form Au caps, which show a novel localized surface plasmon resonance (LSPR). The gold surface on silica spheres modified with a carboxymethyl dextran layer was used for sensing of anti-avidin D with a detection limit of 5.0 nmol·L-1 and a sensitivity of 3.4×10-3 nm·L·nmol-1, which was based on surface-bound avidin molecule. Furthermore, a double stranded DNA (dsDNA) with a binding site of hexa sequence AGGTCA for a nuclear receptor, retinoid X receptor beta (RXRβ), has been successfully immobilized on gold surface via a specific interaction between biotin moiety and streptavidin, and applied to selective recognition of small molecule nuclear ligands like 9-cis retinoic acid.
optical property cap-shaped gold nano-particle biomolecule recognition localized surface plasmon resonance
Zhong Cao Fu-Chun Gong Xi-Xi Huang Shu-Zhen Tan Hiroyuki Takei Juergen Pipper
School of Chemistry and Environmental Engineering Changsha University of Science and Technology Chan Department of Research & Development Life Science Group, Hitachi, Ltd. Hatoyama-machi, Saitama 350-0
国际会议
上海
英文
1596-1598
2008-05-16（万方平台首次上网日期，不代表论文的发表时间）