Spectrophotometric Detection of Dimethyl methyl-phosphonate Using Plant-esterase
We have developed an optical detection method for dimethyl methyl-phosphonate (DMMP) based on reversible inhibition of plant-esterase by monosulfonate tetraphenyl porphyrin (TPPS1). The abosorbance spectrum of the porphyrin-enzyme complex solution is measured with a LAMBDA 500 spectrophotometer. The characteristics of the abosorbance spectrum of TPPS1 are specific and different when the porphyrin is bound to the enzyme. Addition of α-naphthyl acetate or DMMP displaces the porphyrin from the enzyme resulting in reduced absorbance intensity at 420 nm. Absorbance changes at 420 nm show linear dependence on DMMP concentration. DMMP concentrations as low as 9×10-11 mol L-1 have been detected. This optically-based approach promised much simpler and more sensitive detection capabilities.
Plant-esterase TPPS1 DMMP Blue-green region sensor
Changjun Hou Limin Yang Danqun Huo
College of Bioengineering / Key Laboratory of Optoelectronic Technology and Systems of Ministry of E College of of Bioengineering, Chongqing University, China
国际会议
上海
英文
1640-1643
2008-05-16（万方平台首次上网日期，不代表论文的发表时间）