Investigation on diurnal variation and gender difference in human urine using NMR-based metabonomics
1H NMR-based metabonomics has shown considerable potential as a means of rapidly detecting and characterizing a myriad of metabolic components. The 1H NMR spectra obtained for human urine samples were visually inspected and analyzed using principal component analysis (PCA). Urine samples were collected eight times daily (8:00, 10:00, 12:00, 14:00, 18:00, 20:00) from healthy volunteers with a standard diet and lifestyle for 2 consecutive days. Separation of urinary data according to time of sampling collection was achieved. The fluctuations of creatinine, citrate and observed between urine samples collected during the different time of day may be related to hormone levels. In addition, gender-difference was also investigated and the PCA score plots revealed several metabolites that were considered significant for distinguishing between male and female: creatinine, glycine and citrate. These results showed the value of metabonomic methods in the investigation of physiological variation and rhythms, and provided suggestions to collect significative metabonomics data in clinical studies.
1H NMR spectroscopy Metabonomics Diurnal variation Gender difference Principle component analysis (PCA)
Xiaoxia Dai Qiqing Zhang Jiyang Dong Zhong Chen Changqin Liu Shuyu Yang
Research Center of Biomedical Engineering, Xiamen University Xiamen, China Department of Physics Xiamen University Xiamen, China Xiamen First Hospital Xiamen, China
国际会议
上海
英文
2240-2243
2008-05-16（万方平台首次上网日期，不代表论文的发表时间）