Image Processing Technique for Characteristic Test of Cell Based on Electrorotation Chip
On the Basis of image characteristic analysis of Electrorotation (ROT) chip video, the initial cell contour of various movement objectsin each frame have been segmented and each cell contour has been purified to extract using the Snake model, and thenthe projected area and centroid integral of cell etc. have been calculated. Moreover, the Snake cell contour is fitted into an ellipse using the least squares ellipse fitting algrithm. Through omitting the objects whose area, centroid and deflection angle change suddenly with the statistical rule, then the average of the direction (i.e. ellipse deflection angle) difference of the surplus objects between the continual two frames is taken as the rotation vector of the whole cells; The multi-objects are synchronously tracked in each continual dualframes by the frame sequence. Finally the actual rotation speed of each cell is calculated at a specified equal time interval.
Electrorotation Video Snake Model Ellipse Fitting Contour Eztraction Rotation Description
Haiming Li Dianhong Chen Qihua Yang
China Jiliang University Hangzhou,Zhejiang Province, China
国际会议
上海
英文
2548-2551
2008-05-16（万方平台首次上网日期，不代表论文的发表时间）