Ezperimental study on improving nitrogen removal efficiency of denitrify bacteria by protoplast fusion

摘要：

Three bacteria named A1 B1 and C1 were isolated from activated sludge in Zhuankou 863 Experimental Base by limiting dilution and flat crossed separation, which had high nitrogen removal efficiency.Through UV-irradiaion, three single resisitance mutants were obtained, which named K-A1 K-B1 and K-C1.Then these three mutants were used as parent strains to process protoplast fusion and the fusants were selected by medium containing two kinds of antibiotics.Two fusants named CO1 and CO2 were obtained.CO1 was identified to be Bacillus, and CO2 was Nitosococcus.By comparing with their parent strains, the nitrogen removal efficiency of CO1 was 27.4％ higher than C1, and the efficiency of CO2 did not change obviously.

关键词： protoplast fusion denitribatreia fusant nitrogen removal efficiency

作者: Wen-jiao SANG Bei-ping ZHANG Zhen LIU Lei WANG Chun-hua ZHU Lan GAO

作者单位: School of Environmental Science & Engineering, Huazhong University of Science and Technology, Wuhan, School of Environmental Science & Engineering, Huazhong University of Science and Technology, Wuhan, Central Southern China Electric Power Design Institute, Wuhan,Hubei,430071, Peoples Republic of Chi

会议类型: 国际会议

会议名称: The 2nd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2008)(第二届生物信息与生物医学工程国际会议)

会议地点: 上海

会议语种:英文

页码: 3243-3246

在线出版日期: 2008-05-16（万方平台首次上网日期，不代表论文的发表时间）