Study of Transformation and Chromosomal Instability of Human Tracheal Epithelial Cells Induced by Formaldehyde
Objective: To determine the mechanisms of chemical carcinogenesis, the relationship between malignant transformation and genome /chromosomal instability of human bronchial epithelial cell, cell line -BEAS-2B cell exposed to formaldehyde was studied. Methods: The LC50 value of formaldehyde to BEAS-2B cells was tested by MTT assay. Then the cells were induced with formaldehyde at the concentration of 20％ LC50; the characteristics of transformation of cells were identified though the characters of growth curve and colony formation rate of semi-solid agar. The metaphase modal chromosome numbers, karyotypes and nonrandom structural change of induced cell strains were analysed with the G-banding technique. Results: The growth curve of induced cells showed a trend of infinite proliferation and the anchorage independent growth was appeared in 15 generation cells. Before transformed, BEAS-2B cells had a pseudodiploid karyotype and were relatively stable genotypically. After transformed, three characteristic abnormal chromosomes were found. Most of the induced cells were triploid and/or tetraploid cells. The loss of one normal or two homologs of chromosome 14 also had a higher frequency. Furthermore, in their karyotypes, one or two unknown chromosomes appeared and trisomy-5 or trisomy-16 was observed. The induced cells also had a higher frequency of non-stabilizing aberration (39％), including chromosome loss, endoduplication, translocation, break, dicentromere and/or tricentromere etc. Conclusion: Formaldehyde could contribute to progression of transformation and result in genome instability.
formaldehyde human bronchial epithelial cells (BEAS-2B cell) transformation chromosomal instability
ZHANG Gui-zhi CHEN Li XIONG Wei ZHANG Le-feng LI Bian-lan LIU Chang-ting LIU Dong-shan
General Hospital of People Liberation Army Beijing 100853, China School of Public Health and Family Medicine, Capital Medical University, Beijing 100069, China Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China Chinese Center for Disease Control and Prevention, Beijing 100050, China
国际会议
上海
英文
3934-3937
2008-05-16（万方平台首次上网日期，不代表论文的发表时间）