MICRO CUTTING OF MADIN-DARBY CANINE KIDNEY (MDCK) CELL USING FEMTOSECOND LASER

摘要：

Femtosecond Laser is a unique tool for micro and nano fabrication. In this paper, femtosecond laser micro-fabrication is developed as a cell microsurgery technique in micro cutting of living cells filopodia. We have investigated the interaction between femtosecond laser and Madin-Darby Canine Kidney (MDCK) cell, the optimization of micro surgery parameters such as laser energy and exposure time, the designed cutting process of MDCK cells pseudopod. Furthermore, the cells living activity of wound response to cutting was observted and analyzed. Our results demonstrate the feasibility of micro and nano surgery of live cell by femtosecond laser selectivity cutting without compromising the activity of the cells.

作者: Rui Guo Zhen Guo Jianfeng Liu Junjun Ding Xiang Wang Wenhao Huang

作者单位: Precision Machinery and Precision Engineering, University of Science and Technology of China Hefei, School of life sciences, University of Science and Technology of China, Hefei, Anhui, 230026, P.R.Ch

会议类型: 国际会议

会议名称: 第三届太平洋国际激光与光学应用会议(PICALO 2008)

会议地点: 北京

会议语种:英文

页码: 1004-1009

在线出版日期: 2008-04-16（万方平台首次上网日期，不代表论文的发表时间）