Taylor-Made Biomaterials from Collagenic Wastes: Feasible Link between Tanning Industry and Tissue Engineering
The basic aim of the present work is the conversion of non-tanned solid wastes from the tanning industry (splits) into high added value biopolymers for its application in tissue engineering within fields such as cosmetics, medicine or veterinary.From all the previous experience of our team, a specific technology for this kind of waste has been developed. Those tannery wastes have, as an essential feature, a high content of collagen, whose properties and characteristics make this material suitable for a wide range of applications, highlighting their use in tissue engineering. The first stage of the project was centred on finding, through a factorial experimental design, the most suitable treatment for the isolation of biopolymers with optimum properties. The input of this treatment was the extraction of those materials with a minimum hydrolytic effect on the triple helix of the collagen molecule. The following stage was the purification and separation of the biopolymer by molecular weight fractions, using tangential flow ultra-filtration.Those biomaterials could take different forms depending on the future application: 1) gel, 2) film, 3) sponge or 4) fibres. The evaporation of the gels resulted in the formation of films;sponges were obtained by lyophilisation of the samples;and two different kinds of fibres, macro-fibres and nano-fibres were formed through extrusion and electro-spinning, respectively. The formation of these fibres, based on the reconstitution of the collagen structure, can be characterised through optical microscopy (macro-fibres) and electronic microscopy (nano-fibres). All the different samples were thoroughly characterised using calorimetric techniques, microscopy, electrophoresis, mechanical properties analysis and stability.The final stage of the project will be based on the identification of the requirements for the applicability of those new biomaterials. Studies of biocompatibility, toxicity and biodegrability will be carried out for the application of the biopolymers in biomedical fields such as cosmetics, medicine and/or veterinary;more specifically within the tissue engineering in tissue and/or organ regeneration, manufacture of suture fibres, etc.Those taylor-made biopolymers will be designed, with the desired molecular weight and shape, according to their specific future applications.
biopolymer waste fibres
J.Cot M.Catalina P.Celma A.Munich A.Marsal
Consejo Superior de Investigaciones Cientificas (CSIC), C/ Jordi Girona, 18 Barcelona, 08034, Spain Institute Quimico Sarria (IQS), Ramon Llull University, Via Augusta, 390 Barcelona, 08017, Spain
国际会议
北京
英文
257-264
2009-10-11（万方平台首次上网日期，不代表论文的发表时间）