SELECTION OF DNA APTAMERS USING ATOMIC FORCE MICROSCOPY
Atomic force microscopy (AFM) can detect the adhesion or affinity force between a sample surface and cantilever,dynamically. This feature is useful as a method for the selection of aptamers that bind to their targets with veryhigh affinity. Therefore, we propose the Systematic Evolution of Ligands by an Exponential enrichment (SELEX)method using AFM to obtain aptamers that have a strong affinity for target molecules. In this study, thrombin waschosen as the target molecule, and an AFM-SELEX cycle was performed. As a result, selected cycles werecompleted with only 3 rounds, and many of the obtained aptamers had a higher affinity to thrombin than the conventional thrombin aptamer. Moreover, one type of obtained aptamer had a higher affinity to thrombin than the anti-thrombin antibody. AFM-SELEX is, therefore, considered to be an available method for the selection of DNA aptamers that have a high affinity for their target molecules.
C.Ogino Y.Miyachi T.Hayase K.Nosaka A.Kondo
Department of Chemical Science and Engineering, Graduate School of Engineering
国际会议
The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)
北京
英文
8-12
2009-09-17（万方平台首次上网日期，不代表论文的发表时间）