NEW DEVELOPMENT OF LOW-COST AND HIGH-THROUGHPUT DNA SEQUENCING
The newly developed low-cost and high-throughput DNA sequencers are based on the microarray chip platforms on which genomic DNA (gDNA) fragments fixed arbitrarily on the solid substrates and formed as an random gDNA microarray, and high resolution images of fluourescence or chemical illumination were taken from the gDNA microarray during the nucleotide extension process according the gDNA templates. Here, we are now developing a new sequencing route for next generation DNA sequencing. In our sequencing route, the sequenced DNA template array is fabricated by arbitrarily immobilizing single long DNA molecules containing multi-copies of gDNA fragments, and a new strategy of DNA sequencing called as alpha-thiosequencing, has been developed. The sequencing route shows potential to achieve homogeneous amplification in human genome-wide region during preparation of sequenced templates, and high accuracy in high-throughput DNA sequencing.
Lu Zuhong
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
国际会议
The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)
北京
英文
16-17
2009-09-17（万方平台首次上网日期，不代表论文的发表时间）