CONSTRUCTION OF ASYMMETRIC CELL-SIZED LIPID VESICLES FROM LIPID-COATED WATER-IN-OIL MICRODRQPLETS

摘要：

We present a simple, rapid, and robust method for preparing asymmetric cell-sized lipid bilayer vesicles using water-in-oil (W/O) microdroplets transferred through an oil-water interface. The efficiency for producing cell-sized model membranes is elucidated in relation to the vesicular size and the weight of contained watersoluble molecules. We demonstrate the biological asymmetric nature and the formation of lipid raft microdomain structures using fluorescence microscopy.

关键词： Liposome asymmetric membrane microdomain lipid raft

作者: Masahiro Takagi Tsutomu Hamada Mundelanji Vestergaard

作者单位: School of Materials Science, Japan Advanced Institute of Science and Technology,1-1 Asahidai, Nomi, School of Materials Science,Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi,

会议类型: 国际会议

会议名称: The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)

会议地点: 北京

会议语种:英文

页码: 31-35

在线出版日期: 2009-09-17（万方平台首次上网日期，不代表论文的发表时间）