SIMPLE, CHEAP AND PRACTICAL CHIP FOR BIO-ANALYSIS OF POST GENOME AGE
Downscaling of an analytical system can save lab space, reduce expensive reagent consumption and increase the throughput of analysis. All of these are especially attractive for bio-analysis of post genome age. These have been the tasks of lab on a chip technology (or micro-TAS, microfluidics, MEMS) that appeared around 1990, about the same age as another parallel-chip array technology. Nearly 20 years has been past, and great progress has been made. However, the fact is that killing applications are yet to be demonstrated to meet the requirements of front end users. Besides many technical obstacles, cost may be one of the limiting factors. We are trying to show with some examples that how simple cheap and practical chip (SCPC) can be possibly used in bioanalysis of different level, including cell sorting, PCR amplification, DNA, proteins, small molecules and ions. Chip electrophoresis has been one of the main models of typical lab chip, and detector has been the major barrier for system miniaturization. Direct current contact coupled conductivity detector (DC4D) for chip electrophoresis was developed simply based on Ohms law. Sensitive and reproducible separation and detection of small ions were demonstrated. DC4D could be integrated in a chip electrophoresis system without optical length limitation met in optical detection system. Biosensor is very efficient for known specific biomarkers. Using routine photolithographic method, we designed and fabricated glass chips with functional units of passive mixer and sensing electrode, with which electrochemical (EC), chemiluminescence (CL) as well as electrochemiluminescence (ECL) detections can be performed. By immobilizing GOD in the chip, flow electrochemical detection of glucose was successfully demonstrated, and electrochemical interference due to co-existing EC active species was avoided by differential measurement. Super high sensitive flow chip sensors could be developed with the chips by CL or ECL.
Wu Zhiyong
RCAS, NEU, Shenyang 110004, China
国际会议
The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)
北京
英文
47
2009-09-17（万方平台首次上网日期，不代表论文的发表时间）