DESIGN AND PREPARATION OF MONOLAYER-BARCODED NANOPARTICLES PROBE FOR DNA DETECTION
Mass spectrometry (MS) is one of the rapid developed analytical technologies for bio-molecular in recent years. We have developed monolayer-barcoded nanoparticles (MBN) probe for on-chip DNA hybridization assay utilize laser desorption/ionization time-of-flight Mass spectrometry (LDI-TOF MS) (Angeω Chem Int Ed 2008, 47, 5009 -5012), and it was successfully employed in DNA analysis. In this article, we described the details how we designed and prepared MBN probe and demonstrated that the mass-tag molecules of MBN probe can be detected with high sensitivity, in one atto mole (10-18mol) through LDI-TOF MS. And MBN probe also showed acute melt points when mixed with pure oligonucleotide-modified nanoparticles in the presence of target strands which indicate that MBN probe could be used in diagnosing genetic diseases that contain single nucleotide polymorphism (SNP).
Barcoded DNA mass spectrometry SNP sensitivity
Qiu Fei Jiang Dawei Ding Yibing Yang Bing Gu kai Huang Lequn L.
Molecular Medicine Engineering Research Center of the Ministry of Education, and Institute of Molecu School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China School of edicine, Nanjing University, Nanjing 210093, China School of Medicine, Nanjing University, Nanjing 210093, China
国际会议
The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)
北京
英文
71-75
2009-09-17（万方平台首次上网日期，不代表论文的发表时间）