HIGH-THROUGHPUT GENOMIC DNA EXTRACTION FROM BLOOD SAMPLE USING MAGSTATION SYSTEM

摘要：

Automation of nucleic acid extraction and purification, the most difficult step in automating genetic analysis and gene manipulation, can be realized using functionalized magnetic nanoparticles ( MNPs) and automated workstation. For most automated workstation can not be afforded by small lab, we have developed an integrated automation system for semi-automated genetic analysis. The system, Magstation, is equipped with a magnetic separation unit, a heating block and a 96 - well plate holder. Using this system, we could perform the semi-automated extraction of genomic DNA from peripheral blood. The results indicated that it took 25 min to yield 1. 02 to 1. 66 μg genomic DNA from 96 samples using 50 μL blood sample, and OD260/ OD280 ratio was between 1. 78 and 1. 82.

关键词： DNA eztraction magnetic nanoparticles human whole blood genomic DNA

作者: Liu Hongna Li Song Tian Lan Li Zhiyang Ma Chao He Nongyue

作者单位: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering.Southea State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering.Southea

会议类型: 国际会议

会议名称: The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)

会议地点: 北京

会议语种:英文

页码: 84-86

在线出版日期: 2009-09-17（万方平台首次上网日期，不代表论文的发表时间）