DESIGN AND APPLICATION OF THE SOFTWARE FOR COUNTING COLOR-BEADS RANDOMLY DISTRIBUTED ON THE CHIP
The appearance of gene chips and many other new technologies is reshaping the biology research and gene chips have been widely applied in molecular diagnosis. Some gene chips use micro-beads which include various micro-particles. Normally these chips immobilize micro-beads irregularly for the sake of cost and convenience. How to analyze subsequent images and get useful information from them has become an essential prerequisite for quantitative analysis. We designed software to analyze images of this kind of gene chips and counting the number of signal points. The software is applied in analyzing images by scanning the gel-chips immobilized with the mixed beads hybridized with fluorescent probes labeled by Cy3 and Cy5. Contrasts of results respectively coming from the software and manual work were did, which could prove the results were satisfying.
Micro-bead chip digital counting bioinformatics image processing
Li Cheng Sun Xiao Huang Huan Zhou Guohua
Huadong Research Institute for Medicine and Biotechnics, Nanjing 210002, China Key Laboratory of Mol Key Laboratory of Molecular and Bimolecular Electronics (Southeast University) Ministry of Education Huadong Research Institute for Medicine and Biotechnics, Nanjing 210002, China
国际会议
The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)
北京
英文
103-105
2009-09-17（万方平台首次上网日期，不代表论文的发表时间）