A NOVEL KIND OF POROUS PSEUDO-CARBON PASTE ELECTRODE ELECTROCHEMICAL BIOSENSOR AND ITS APPLICATION INBIOASSAY

摘要：

A kind of novel and sensitive porous pseudo-carbon paste electrodes (PPCPEs) were directly fabricated by mixing organic microspheres or inorganic microspheres as templates, graphite powders as the filler, pyrrole as the precursor of polymer which actually acts a net to keep the fabricated electrode as an integrated one without addition of any organic pastes. The fabricated PPCPEs were investigated by cyclic voltammetry, and it was shown that the electrochemical signal of PPCPE was the strongest among those three kinds of electrodes. The DNA hybridization reaction on PPCPE was monitored by anodic stripping voltammetry (ASV) analysis with the help of gold nanoparticle-catalyzed silver enhancement and the peak current of Ag at PPCPE was linear with the amount of complementary oligonucleotide ranging from 1 to 80 nM and 0. 08 to 1 nM, the detection limit was as low as 0. 05 nM

关键词： Porous pseudo-carbon paste electrode pyrrole carbon nanotube modified carbon paste electrode, DNA electrochemical biosensor.

作者: Xu Lijian Deng Yan Li Song Du Jingjing He Nongyue

作者单位: The Hunan Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Zhuzhou 41 The State Key Laboratory of Bioelectronics, Southeast University, China The Hunan Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Zhuzhou 41

会议类型: 国际会议

会议名称: The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)

会议地点: 北京

会议语种:英文

页码: 111-113

在线出版日期: 2009-09-17（万方平台首次上网日期，不代表论文的发表时间）