DEVELOPMENT OF AN SEMI-AUTOMATED SYSTEM WITH A MAGNETIC NANOPARTICLES SEPARATION DEVICE FOR SNPS DETECTION

摘要：

We have developed an integrated automation system for genetic analysis and gene manipulation. The system, MagStation- A, is equipped with a magnetic separation unit, a heating block, power supply and a control system. Semi-automation of nucleic acid extraction and purification, the most difficult step in automating genetic analysis and SNPs genotyping manipulation, was realized using magnetic particles (MPs) and solid phase single base extension (spSBE), which we have previously developed for automating the handling of MPs. Using this system, the methylenetetrahydrofolate reductase (MTHFR) gene C677T polymorphism of 48 different samples was semi-automated integrated.

关键词： Magnetic particles automated MagStation sing base eztension high-throughput single nucleotide polymorphisms

作者: Li Song Liu Lishang Liu Hongna Tian Lan Li Zhiyang HeNongyue

作者单位: Key Laboratory of Green Packaging and Application of Biological Nanotechnology of Hunan Province, Hu Key Laboratory of Green Packaging and Application of Biological Nanotechnology of Hunan Province, Hu State Key Laboratory of Bioelectronics, School of Biological Science and Medical ngineering, Southea State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southe Key Laboratory of Green ackaging and Application of Biological Nanotechnology of Hunan Province, Hun

会议类型: 国际会议

会议名称: The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)

会议地点: 北京

会议语种:英文

页码: 118-121

在线出版日期: 2009-09-17（万方平台首次上网日期，不代表论文的发表时间）