FLOW SOLID PHASE PYROSEQUENCING REACTION ON CAPILLARY MICROFLUIDIC CHIP

摘要：

A simple flow solid phase pyrosequencing reaction system was introduced with a microfludic chip consisted of fused silica capillary and permanent magnets. The magnetic beads bearing the template ssDNA were magnetically immobilized in the capillary, and the reaction solutions, including washing buffer and detection mixtures containing the bases, enzymes (polymerase, ATP sulfurylase and luciferase) and substrates (adenosine 5-phosphosulfate and luciferin) were sequentially aspirated by a syringe pump through the EP tubes installed on a turning wheel. The signal was effectively enhanced by bioluminometric assay coupled with modified primer extension reactions (BAMPER) in the single-nucleotide polymorphisms (SNP) assay. SNPs typing of rs8130833 and rsl053315 from human chromosome 21 were demonstrated, and the results were in agreement with the established methods.

关键词： Microfluidic solid phase pyrosequencing reaction magnetic beads magnetic immobilization single-nucleotide polymorphisms (SNPs) bioluminometric assay coupled with modified primer eztension reactions (BAMPER)

作者: Wu Zhiyong Qu Baiyan Huang Huan Ren Qianqian Wu Haiping Zhou Guohua

作者单位: Research Center of Analytical Sciences, Northeastern University, Shenyang 110004,China Research Center of Analytical Sciences, Northeastern University, Shenyang 110004, China Huadong Research Institute for Medicine and Biotechnics, Nanjing 210002, China

会议类型: 国际会议

会议名称: The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)

会议地点: 北京

会议语种:英文

页码: 129-131

在线出版日期: 2009-09-17（万方平台首次上网日期，不代表论文的发表时间）