MICROCHIP FREE FLOW ELECTROPHORESIS (μ-FFE) FOR ISO-ELECTRIC FOCUSING (IEF)

摘要：

A microfluidic chip for free flow electrophoresis was prepared simply by glass or PMMA plates with double tape. The separation channel was isolated from the electrolyte reservoirs by positioned photo-polymerization of conductive gel. Continuous iso-electric focusing (IEF) fractionation of a model protein mixture was demonstrated and collected in different outlet reservoirs, followed by capillary electrophoresis characterization. Monolithic bed was synthesized in situ in the separation channel, and a pH gradient was established with ampholine by applying voltage across the channel, and the ampholine was covalently immobilized locally on the bed as shown by a color indicator. Preliminary result shows that gel and ampholyte free chip FFE IEF fractionation is possible, which is very important for proteomics analysis.

关键词： Chip free flow electrophoresis (FFE) iso-electric focusing (IEF) protein, monolithic separation channel pH gradient immobilization

作者: Li Ying Meng Xiangwen Fang Fang Wu zhiyong

作者单位: Research Center of Analytical Sciences, Northeastern University, Shenyang 110004, China

会议类型: 国际会议

会议名称: The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)

会议地点: 北京

会议语种:英文

页码: 135-137

在线出版日期: 2009-09-17（万方平台首次上网日期，不代表论文的发表时间）