NEW NANOFLUIDIC INTERFACE FOR ONLINE PRE-CONCENTRATION OF CHARGED SPECTIES IN CAPILLARY CHIP ELECTROPHORESIS

摘要：

By HF etching the outside wall of fused silica capillary, ion conductive fused silica membrane with pores of nanometer scale was formed and used as a new nanofluidics interface. By voltage bias with anode inside, rhodamine 6G (1. 2 nm) in 1 mM pH 8. 5 Tris-HCl inside the capillary was pre-concentrated on the cathode side (outside), and fluorescien (0. 7 nm) was also found accumulated at the cathode side, indicating a typical concentration polarization effect of nano ion channels. Neutral rhodamine B (1. 6 nm) was not found permeable through the membrane, indicating that the pore size is of nanometer scale. It is shown here that, this membrane could not only be used for electro-concentration of protein and dsDNA molecules, but also for small charged molecules. This membrane is easy to prepare, rigid, transparent, and multiple applications could be developed as an online sample processing unit in chip electrophoresis.

关键词： Nanofluidics chip electrophoresis fused silica membrane online pre-concentration Dornnan effect concentration polarization

作者: Wu Zhiyong Li Tingting He Yanqin Fang Fang

作者单位: Research Center of Analytical Sciences,Northeastern University, Shenyang 110004, China Research Center of Analytical Sciences, Northeastern University, Shenyang 110004,China Research Center of Analytical Sciences, Northeastern University, Shenyang 110004, China

会议类型: 国际会议

会议名称: The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)

会议地点: 北京

会议语种:英文

页码: 138-140

在线出版日期: 2009-09-17（万方平台首次上网日期，不代表论文的发表时间）