THE DIFFERENTIATION OF EMBRYOID BODIES INTO CARDIOMOYOCYTES USING A MICROFLUIDIC CHIP
Embryonic stem (ES) cells are known to differentiate into other mature cell types through the formation of embryoid bodies (EBs). The various microenvironment of EBs is so crucial for the procedure of differentiation that the microfluidic chip offers a potential platform for high-throughput testing without much consumption of reagents. Here, we designed a three layer microfluidic chip with micro-structures that created relatively uniform microenvironment for EBs culture. The EBs were loaded into the microfluidic chip and then perfused by vitamin C (VC) for six days. The results suggest that EBs could spread and have the trend of differentiation into cardiomoyocytes.
Embryoid bodies microfluidic chip cardiomoyocytes
Ai Xiaoni Liang Qionglin Wang Yiming Luo Guoan
Key Laboratory of Bioorganic Phosphorus Chemistry &.Chemical Biology (Ministry of Education), Depart Key aboratory of Bioorganic Phosphorus Chemistry &.Chemical Biology (Ministry of Education), Departm
国际会议
The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)
北京
英文
154-155
2009-09-17（万方平台首次上网日期，不代表论文的发表时间）